Mouse 5'-Nucleotidase Ecto ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse 5'-Nucleotidase, Ecto in biofluid samples, such as serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . NT5E present in samples reacts with an anti-Mouse NT5E capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-NT5E detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active NT5E in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse NT5E. Detection Target: Mouse 5'-Nucleotidase, Ecto Reactivity: Mouse Range: 78.1-5000pg/mL Sensitivity: 26.1pg/mL Storage Conditions: Store all components at 4┬░C This Mouse NT5E activity assay is for the quantitative determination of 5'-Nucleotidase, Ecto in samples including serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.