Mouse Nephrin ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Nephrin in biofluid samples, such as urine, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . NPHN present in samples reacts with an anti-Mouse NPHN capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-NPHN detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active NPHN in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse NPHN. Detection Target: Mouse Nephrin Reactivity: Mouse Range: 0.312-20ng/mL Sensitivity: 0.123ng/mL Storage Conditions: Store all components at 4┬░C This Mouse NPHN activity assay is for the quantitative determination of Nephrin in samples including urine, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.